Part:BBa_K515001

IaaH - Indoleacetamide hydrolyase

Indoleacetimide hydrolase catalyses the hydrolysis of indoleacetamide to indoleacetate and ammonia. The enzyme originates from the plant pathogen Pseudomonas savastanoi which produces the auxin, indole-3-acetic acid via a two step pathway.

This is one of the parts of our composite auxin expressing construct, BBa_K515100.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1052
  
• 1000
  COMPATIBLE WITH RFC[1000]

This BioBrick has been sequence verified.

For the full characterisation of the device, please refer to the BBa_K515100 page.

Contribution: NUDT_CHINA 2015

Author: Xinyuan Qiu

Summary: We built a new part based on this part to extend its function.

We designed a new part by removing the RBS from this part to extend its usage.

A new Bio-brick was designed based on this part

Primers used to build the new part:

F-Prime: 5’- GGAATTCGCGGCCGCTTCTAGAGATGCGCGAAATG -3’

R-Prime: 5’- GCGGGCGGCGGACTAGTCTTATTAGCCTTTTAACAC -3’

For further information,

See BBa_K1789001

This part was sent to the registry.